The study on disturbance behavior of subsoiling is the basis to understand the subsoiler-soil interaction law deeply. A working model of subsoiling was established by using discrete element method. In addition, with the aid of highspeed photography technique and the indoor soilbin test, the micro movement and the macro disturbance behavior of the soil at different positions were compared and analyzed. The results showed that, the disturbance range of soil was decreased with the increasing distance between the soil and the subsoiler, and at different locations, the disturbance range from large to small was the shallow layer, the middle layer and the deep layer. In the subsoiling range, the movement speed of the soil was decreased with the increase of the distance between the soil and the subsoiler. The distribution curves of the samespeed soil particles were basically consistent with the curves of the subsoiler. The average velocities of soil particles in different depths and different directions were measured from large to small as follows: the shallow layer, the middle layer, the deep layer in x direction; the middle layer, the shallow layer, the deep layer in y direction; and the deep layer, the middle layer, the shallow layer in z direction. The discrete element simulation could accurately simulate the disturbance behavior of subsoiling, and the shape of soil disturbance section of simulation and obtained from experiment were basically matched. The relative error of the soil bulkiness and soil disturbance coefficient between simulation and experiment were 13.21% and 17.38%, respectively. The relative error between the simulated longitudinal accumulation angle of the surface soil and that of experiment was 9.42%.