To provide theoretical reference for confirming light properties of locust’s directive selection and screening combination the different light field in locust capturing machine by photoelectric equipment, the discrepant comparison that locust’s directional response to polarized blue light with different illumination and unpolarized light with different spectrum was investigated through the comparative test by means of LED unpolarized light, polarized blue light and phototaxis behavior trial equipment, and the principle of the locust’s directive selection influenced by polarized blue light and unpolarized light was discussed. Under the same illumination of light source, photoelectric simulation generated by polarized blue light and unpolarized light could inspire locust’s biophotoelectric effect, while the sensitive degree of locust on spectrum and polarized blue light’s illumination determined the difference of locust’s directional response to polarized blue light and spectral light. When the illumination of polarized blue light did change, locust’s directional response to linear polarized light was the best. Comparing the different illumination of polarized blue light with locust’s sensitive spectrum, locust’s phototactic response to violet light was the best. Relative to linear polarized light, the alternating illumination of polarized blue light did not enhance locust’s phototactic response. Considering about light properties of the effect on locusts induced, locust’s sensitive spectral illumination on improving locust’s phototactic inductive rate was a key factor.