The aim was to use a non-destructive rapid monitoring method to timely reflect the physiological reaction and detection the response of rice leaves caused by heavy metals. A potted rice experiment was used, which treated with three kinds of exogenous heavy metals ions (copper, zinc, nickel) in soil, and four levels of heavy metal content were set. The electrical impedance spectroscopy technique can be employed for quickly diagnosis of the nutritional status of plants and serves as a non-destructive diagnostic method in agriculture. Therefore, rice leaves treated for 21 days were subjected to EIS analysis, followed by measurement of physiological response indexes to establish the correlation between physiological indexes and EIS parameters. Experimental results demonstrated a strong correlation changes in the electrical impedance Re/Ri of rice seedling leaves and electrolyte leakage rate, MDA levels, as well as leaf selection. Furthermore, under copper (Cu) treatments, the Re/Ri values of the first unfolding leaf at the top exhibited a higher correlation with MDA levels and electrolyte leakage rate, reaching coefficients of 0.956 and 0.938. Similarly, after zinc (Zn) treatment, the Re/Ri values of the second unfolded leaf showed significant correlations with MDA levels and electrolyte leakage rate, achieving coefficients of 0.944 and 0.969 respectively. Additionally, following nickel (Ni) treatment, both the first and second unfolded leaves displayed high correlations between their impedance values and MDA levels, proline content as well as leakage rate. In summary, these findings indicated that measuring Re/Ri in rice leaves can serve as an effective non-destructive method for rapidly assessing heavy metal stress on rice plants by reflecting various physiological indicators.