Negative pressure irrigation technique, which is one of the new irrigation technology for saving water, was applied to substrate culture in greenhouse. There were fewer researches on the relationship between the daily consumption of tomato and meteorological factors added to the leaching link in substrate culture. The daily consumption of plant was affected by the interaction with meteorological factors and by the sensitivity of the meteorological factors. It was beneficial to develop the system of supplying nutrient solution and the management methods with substrate culture using negative pressure in greenhouse based on mastering above relationships. Tomato was used as a material with substrate culture testing for the condition of negative pressure to supply nutrient solution. The daily consumption of nutrient solution and meteorological factors, e.g., air relative humidity, air temperature and solar radiation intensity, were measured. Based on these factors, a multivariate regression model was established and the direct path coefficient, indirect path coefficient, determination coefficient and total contributions for estimating the reliability degree of the regression model were calculated by path analysis method. The results showed that the direct path coefficients of crop coefficient and daily average solar radiation intensity reached a very significant level (P<0.01). The determination coefficient of these two factors and the contributions to R2 among the factors were the maximum, thus the two factors were the important meteorological factors on daily consumption of nutrient solution for tomato.From the effect of meteorological factors reduction on daily consumption value by path analysis, it was indicated that the daily average solar radiation intensity was the strongest sensitive factors, followed by the crop coefficient and daily maximum solar radiation intensity.