As the development of breeding technology, the transgenic crops of single-character were not able to meet the demands of modern agriculture, and the stacked transgenic plants that combine different characters have gradually raised the public’s attention. Based on the literature review, some detection methods of stacked transgenic plants were introduced. The first detection technology was based on the protein, including lateral flow strip detection technique and ELISA detection technology;the second one was based on nucleic acid detection technology, including PCR detection technology, gene-chip technology, isothermal amplification technology and next-generation sequencing technology. In addition, the traceability technology of genetically modified crops and their products was also introduced. The results indicated that the transgenic detection technology was gradually developing to high throughput, high accuracy and high sensitivity and some new detection technologies had appeared and developed;the integration of computer technology and information technology would contribute to the traceability system building;the developing trend of the traceability technology would inevitably need to combine a variety of information technologies in order to integrate the advantages of them.