In order to solve the problems relating to pesticides detection with the use of complex instruments in terms of high cost, tedious operation and low degree of automation, a new method for pesticides detection with paper-based microfluidic chip was provided. The paper-based microfluidic chip was designed and developed with automatic sample injection, hybrid reaction and electrochemical detection. Subsequently, the enzyme electrode of ring structure was prepared by chemical crosslinking method using graphite carbon and Ag/AgCl materials and then characterized with electrochemistry cyclic voltammetry (CV). The integrated enzyme electrode of the paper-based microfluidic detection system for pesticides was built based on enzyme inhibition mechanism. Finally, the linear model between inhibition rate and parathion pesticides concentration was set up and the performance of the enzyme electrode was tested. The results showed that the prepared enzyme electrode was of good repeatability, linearity and stability. Furthermore, a linear relationship was displayed between inhibition rate and parathion pesticides, which was represented by the equation I=158.82+21.11lgC with good linear range of 1.0×10-7g/mL~1.0×10-5g/mL. The determination coefficient obtained was 0.993 and the corresponding limit of detection was 3.3×10-8g/mL. The enzyme electrode integrated on the paper-based microfluidic chip was robust with parathion pesticides which can not be interfered with other pesticides and substance with standard addition recovery rate of 95.8%~115.0%.