Crude polyphenols were extracted from two types of grape skins and two wines. Total antioxidant activities and in vitro DPPH free radical scavenging activities of the extracts were evaluated. Meanwhile, the effect of those extracts on angiogenesis inhibition was investigated by using transgenic zebrafish model. After zebrafish embryos were treated with the crude polyphenol extracts for 22h, the inhibitory effect on the intersegmental vessel (ISV) of zebrafish was investigated. The results indicated that the wines and grape skins all had in vitro antioxidant activities. However, the DPPH free radical scavenging activity and total antioxidant activity of wines were higher than those of grape skins. Wine 1 had the highest DPPH free radical scavenging activity (89.42%), which was 1.29 percentage points higher than that of wine 2. Wine 2 had the highest total antioxidant activity of 223.24U/mL. Compared with control groups, zebrafish treated with crude polyphenol extracts of wines had significantly less ISV formation. Wine 1 had better inhibitory activity on ISV and dorsal longitudinal anastomotic vessel (DLAV) formation. However, the crude polyphenol extracts of grape skins showed little inhibitory effects on ISV and DLAV formation of zebrafish.