For no full analysis on the theories of three planting bars of transplanting mechanism and reducing the row spacing applicable range by increasing the quantity of planting bars, a non-uniform rotation three planting bars of transplanting mechanism was proposed. The kinematics analysis model was built based on its working principle, and the effects of transplanting parameters on non-uniform rotational speed were studied. The theoretical analysis was received that the non-uniform rotational speed had no effects on the static trajectory of planting hoe, taking seedling angle, pushing seedling angle and trajectory height. Eccentricity ratio and initial phase angle of the elliptic gears had great effects on the velocity with axis y at the point of taking seedling, the speed of taking seedling points with axis y reached the largest when the phase angle was 0°. The transplanting hole was increased with the increase of eccentricity ratio, the initial phase angle had relatively complex effects on the transplanting hole, which would get large with the increase of initial phase angle between 0°~90°, and get small with the increase of initial phase angle between 90°~180°. The effects of speed of transplanting bars and the movement times of seedling box on the damaged seedling rate and stripping-slicing homogeneities were tested in laboratory, the results indicated that the damaged seedling ratio was increased with the increase of revolving speed and the impact was significant, but the movement times had no significant impact on it. The movement times had significant impact on the stripping-slicing homogeneity, which was significantly higher than the matched group when the movement times was 18, the difference between the stripping-slicing homogeneities when the movement times equaled to 21 and 24 and the two contrast transplanting bars was little, but the revolving speed had effect on it and the impact was non-significant. Field contrast test indicated that the planting quality of the non-uniform rotation three planting bars was superior to that of the matched group, the damaged seedling rate was increased with the increase of work speed, the laid seedling hill rate and the floating seedling rate were also increased with the increase of work speed, but the impacts were non-significant, and the work speed had no effect on the missing planting hill rate.