Pneumatic ejector is an important vacuum source for agricultural automation. During the application of pneumatic ejector, the increasing back pressure may cause the suction ability to get worse intensely and the normal operation of the system will be greatly influenced. Regarding the ejectors used in pneumatic systems, the air flow was analyzed with finite volume method based on ejector inner mechanical structural parameters. The changes of pressure distribution and suction flow velocity were studied when the back pressure was variable. Based on the theoretical analysis, an experimental apparatus was set up and the suction mass flow rate was measured as the back pressure increased. The relationship between back pressure and suction mass flow rate was obtained. Judging from both of theoretical analysis and experimental result, the suction flowrate characteristics remained unchanged at first and then got worse intensely as the back pressure increased. A method was proposed to prevent reverse flow by estimating back pressure, which was an effective complementary approach for ejector systematic design. When designing a pneumatic ejector system, the back pressure range where the ejector worked normally should be derived both from theoretical calculation and experimental test. Reverse flow could be prevented if the back pressure was controlled within normal range, thus the optimum performance would be achieved.