Aiming at the problem of multi-objective design for refrigerated compartment, an optimal design model of refrigerated compartment was established. The actual constraints of compartment heat transfer, compartment seal, respiratory heat of goods within the refrigerated compartment and its main design parameters were comprehensively considered. The smallest heat transfer coefficient and maximum internal space of compartment were selected as objective function. Parameters of the refrigerated compartment were optimized with Matlab software, and the compartment heat transfer coefficient and the best thickness of compartment insulation material under different parameters were analyzed. Results showed that the optimized design method was suitable for insulated compartment of refrigerated trucks. The optimized heat transfer coefficient and the smallest thickness of compartment insulation material were different under different conditions. When truck speed was zero and the thermal conductivities of compartment insulation material were 0.007, 0.023, 0.030, 0.042, 0.045W/(m·K), and the conditions of optimal compartment heat transfer coefficient and the largest internal space of compartment were satisfied simultaneously, the corresponding optimal thicknesses of compartment insulation material were 0.07, 0.14, 0.16, 0.19, 0.20m, and the heat transfer coefficients were 0.0985, 0.1603, 0.1823, 0.2139, 0.2176W/(m2·K), respectively. The thickness of compartment insulation material decreased and compartment heat transfer coefficient increased with the increase of truck speed. In addition, there was a positive correlation between the smallest thickness of compartment insulation material and the optimal heat transfer coefficient.