The study aimed to investigate the effects of exogenous microorganisms on fermentation efficiency of Sophora flavescens branches for substrate production. The randomized block design was used with dried chicken manure as the nitrogen source. The effects of exogenous microorganisms on fermentation performance parameters of Sophora flavescens branches in substrate production were studied. The results showed that the vaccination crude fiber degrading bacteria inoculation and cellulose enzyme preparation, which had a persistent high temperature (higher than 50℃ and reached to 5d) and a shorter body decomposition time of Sophora flavescens, had a significant temperature increasing speed which faster than CK. At the end of the pile, for the vaccination crude fiber degrading bacteria inoculation and cellulose enzyme preparation, the TOC decreased by 8.52% and 8.01%, C/N ratio decreased by 5.35% and 5.00%, content of cellulose decreased by 3.31% and 3.29%, degradation rate of hemicellulose decreased by 2.89% and 2.93%, lignin content decreased by 2.01% and 1.98%, total nitrogen increased by 16.1% and 18.2%, total phosphorus increased by 18.5% and 19.0%, total potassium increased by 49.1% and 48.1%, respectively, which accelerated the decomposition of organic matter and cellulose, improved the content of total nitrogen, total phosphorus and total potassium and ensured the fertility after maturity. Moreover, with the vaccination crude fiber degrading bacteria inoculation and cellulose enzyme preparation, the pile density increased by 6.10% and 9.20%, the total porosity increased by 9.28% and 9.90%, the water holding porosity increased by 4.83% and 6.09%, respectively. After the maturity, the physical and chemical indicators were in line with the requirements of an ideal matrix. The germination index (GI) of cabbage and cucumber seed were up to more than 85%, eliminating the toxic effects of Sophora decomposing products effectively. There was no significant difference between the performance parameters of vaccination crude fiber degrading bacteria inoculation and cellulose enzyme preparation. Considering the demanding of fermentation temperature, decomposition cycle, crop protected plant root growth and fixed function of the physicochemical properties, the vaccination crude fiber degrading bacteria inoculation and cellulose enzyme preparation had a good promoting effect on Sophora flavescens substrate.