Temperature mismanagement and physical damage are considered as the most common causes for postharvest losses of fresh fruit. The partition packaging and common packaging without partitions were taken as research objects. A three dimensional mathematical shear stress transport (SST) k-ω model of computational fluid dynamics (CFD) was established to simulate the temperature and velocity field distribution of partition packaging and common packaging under the same boundary condition and the same initial condition. Compared with cooling time, cooling rate and cooling uniform of two packages, the partition package and common package were basically the same, the difference of cooling temperature was not more than 0.67℃, the maximum difference of cooling rate was 0.012℃/min, and the coefficient of temperature variation of partition packaging was slightly larger than that of common packaging temperature. The two kinds of packaging had the same precooling effect and met the precooling requirement in cold chain transport. Compared with experimental results, the root mean square error of two packaging results was 1.13℃ and the average relative error was 8.2%, which proved the rationality and accuracy of simulated result. The research result was of great significance for the relationship between balance temperature loss and physical damage.