To explore the effects of biochar and fertilizer interaction on tomato soil moisture content and yield, five biochar levels were set at 0t/hm2 (B1), 10t/hm2 (B2), 20t/hm2(B3), 40t/hm2 (B4) and 60t/hm2 (B5); two fertilization levels were set at midfertilizer (F1) and lowfertilizer (F2). The results showed that with the increase of biochar application, the soil water content of the highbiochars were increased. Among them, B4F1 was the most significant and was increased by 40%. However, the soil water content in 20~40cm was opposite to that of 0~20cm. Compared with the control, it showed a downward trend. Among them, B4F1 and B4F2 had the smallest soil water content, which was only 70% of the control. The overall changing trend of soil water content in 40~80cm was consistent with that in 20~40cm. After the application of biochar, the changing range (Ka) and variation degree (Cv) of soil water content were decreased. For the same depth of soil, with the increase of application rate, the biochar Ka and Cv appeared decreasing trend. Compared with the control, the Cv under high application of charcoal (B4F1, B4F2, B5F1 and B5F2) was relatively small. With the growth of tomatoes, the effect of soil water content on the vertical profile showed that compared with the control the higher application of biochar (B4F1, B4F2, B5F1 and B5F2) effectively maintained the effective water. With the increase of biochar application, the increase in tomato yields was firstly increased and then decreased, and both were higher than that of control. The yield of B4F1, B4F2, B5F1 and B5F2 were increased by 46.34%, 58.61%, 49.63% and 39.18%, respectively. Among them, the yield of B4F2 treatment was the highest. There was no significant difference in different fertilization treatments. The research results can provide a basis for agricultural production in semiarid regions of Inner Mongolia.