The Shewanella separated from corruption large yellow croaker was used as research object. The growth data of Shewanella putrefaciens under different pH values, NaCl concentrations and temperatures was obtained by using viable count and optical density methods, and the Gompertz model was built. Effect of environmental factors on growth kinetic parameters was compared and analyzed. Results showed that Shewanella putrefaciens could not grow at pH value of 6.0 and NaCl concentration of 3.5% during 3~8℃. The differences of maximum specific growth rate (μmax) and generation time were not significant (p>0.05), but the differences of lag phase (λ) and the most probable density (M) were significant (p<0.05). Environmental factors had significant effect on the growth kinetic parameters.High temperature or low NaCl concentration resulted in short generation time. When temperature was 3~25℃, high temperature resulted in large μmax. The increase rate of μmax was (0.0143±0.0032)h-1/℃.When temperature was high, λ would be short. The λ was decreased at a rate of 1.5h/℃ by using viable count method, while during 8~15℃, it was decreased approximately at a speed of 2.5h/℃ by optical density method. The μmax was influenced significantly by NaCl concentration and pH value, while lag time showed no significant changes by viable count method. Using optical density method, changes of μmaxwere affected unsignificantly by NaCl concentration and pH value, while changes of λ were apparent. Thus, both of the two counting methods could judge the growth/nongrowth state of Shewanella, and the estimation errors of λ and M were increased by harsh environments when using optical density methods.