In order to increase the yield of intertilled crops and enhance the effective utilization rate of fertilizer, a kind of corn automatic hole fertilization control mechanism for intertilled crop was designed. The structure and working parameters of hole fertilization control mechanism were analyzed by soil bin testing. The influence law of parameters of transmission mechanism on the accuracy of fertilizing control was studied. The orthogonal rotation combination method was used with the power output shaft speed, vehicle forward speed and seedling needle height as impact factors, the seedling injury rate and the fitting degree of holes and plants as response functions. The regression analysis of Design-Expert platform and the response surface method were used. The singlefactor and multifactor orthogonal experiment was carried out. The results showed that, when the vehicle forward speed was 1.25m/s, power output shaft speed was 16.5r/min, seedling needle height was 5mm, the fitting degree of holes and plants can reach to 0.796 and seedling injury rate was close to 10%. The optimized parameters can meet the requirements of corn fertilization during seedling stage.