An electrode for the detection of rutin in tea and pill was produced and fabricated, which was modified by the glass carbon electrode (GCE) surface by graphene (GR) nanometer materials and n-octylpyridinum hexafluorophosphate (OPFP). Different electrochemical methods such as cyclic vohammetry, impedance spectroscopy and square wave voltammetry were used to investigate the electrochemical behaviors of rutin on IL/GR/GCE, indicating that greatly improved the electrochemical performance and detection response due to ionic conductivity and adhesiveness of room temperature and the large specific surface area and high electron transfer of graphene. Under the optimal conditions using square wave voltammetry, the rutin oxidative peak current was linearly related to its concentration in the range from 0.05μmol/L to 11μmol/L with the detection limit estimated to 0.01μmol/L(S/N=3) and the linear regression coefficient was 0.997. Finally, the developed electrode was successfully applied to detect the content of rutin in real samples, which displays good stability and excellent anti-interference ability.