The soil separation device, composited with vibration unit and transformation unit, was designed by using AIP software. The proposed device was assembled at the type QM-500 fruit seedling lifter came with a 58.8kW tractor. The experiments were carried out in the fruit seedling breeding base of Lixian county. Experiments showed that when the eccentric distance was 52mm, the tilt angle was 13.72°, the coefficient of travel speed variation was 1.02, the polar angle was 2.03°. The field experiments showed that the lifting efficiency was 1.6 plants per second. The manpower was decreased by 50.85% which reduced the labor intensity and realized the simplified operation of the apple seedling lifter.