The effect of hypobaric storage under different pressures on the energy metabolism of juicy peach fruit were studied. The pressures were controlled at (10±5) kPa and (80±5) kPa, respectively with air storage as control. Contents of ATP, ADP, AMP and energy charge, and the activities of mitochondria respiratory metabolism-related enzyme H+-ATPase, Ca2+-ATPase, succinic dehydrogenase (SDH), cytochrome C oxidase (CCO) were determined during storage. The results indicated that the hypobaric storage significantly maintained higher H+-ATPase, Ca2+-ATPase, SDH and CCO activities, and inhibited the decreases of contents of ATP and energy charge level. Hypobaric storage under (10±5) kPa pressure was more conducive to maintain energy level and the activities of mitochondria respiratory metabolism-related enzyme. These results suggested that the benefits of hypobaric storage associated with energy metabolism.