In order to separate and purify 6-gingerol from oleoresin by macroporous resin, 6 kinds of macroporous resins were chosen to carry out static and dynamic adsorption-desorption experiments. Qualitative and quantitative analysis of purification results were analyzed by HPLC-UV and GC-MS. The results showed that D101 was the optimal macroporous resin for separating and purifying 6-gingerol, the best technology parameters were as follows: sample concentration of 2 mg/mL, distilled water of 3 BV, 80% ethanol for elution velocity of 2 mL/min, elution volume of 4BV. Under these conditions, the purity of 6-gignerol enhanced from 1.54% to 71.32%.