The microbial pretreatment of yellow corn stover and corn stover silage was achieved via the solid state cultivation of white rot fungi. Pretreatment effects on the biodegradability and subsequent anaerobic production of methane were investigated. The white rot fungi pretreatment of yellow corn stover was accompanied by 38.3% cellulose, 42.2% hemicellulose, and 39.1% lignin degradation, while the degradation of corn stover silage was 9.9% cellulose, 23.2% hemicellulose, and 15.2% lignin. The reducing sugar yield of pretreatment yellow corn stover and corn stover silage after enzymatic hydrolysis was 195.8 mg/g and 67.7 mg/g respectively. These indicated much more composition of yellow corn stover was degradated, and more available composition retained in the corn stover silage after pretreated.The results showed that the total VS methane production of pretreated corn stover silage was 215.5 mL/g after 21 d anaerobic digestion,which increased by 29.2% more than the untreated ones and accounting 73.1% for 60 d total methane production. The biogas production of pretreated yellow corn stover was not increased. So microbial pretreatment of corn stover silage by white rot fungi could increase substrate biodegradability, and further stimulate methane production.