In order to study the binding characteristics of cuticle and prism layer of Cyclina sinensis and apply it to improve the anti-corrosion performance of agricultural machinery, type-JSM—6700F scanning electron microscope and the revetest scratch tester of CSM instruments Revetest Xpress Plus were used to observe the surface microstructure and binding characteristics of cuticle and prism layer of Cyclina sinensis, respectively. The results showed that the binding characteristics of cuticle and prism layer was better when the spherical particles size of the back of Cyclina sinensis cuticle layer and the bore diameter of the prism layer surface were in the same range. The scratches of different parts of Cyclina sinensis cuticle surface existed a certain difference. The fluctuations of friction coefficient were large when horizontal scored and they were small when longitudinal scored. The results indicated that the binding characteristics of cuticle and prism layer was relatively better, especially the binding characteristics of cuticle and prism layer of Cyclina sinensis abdomen.