The pre-cooling process of Agaricus bisporus by using heat transfer theory was analyzed. The cooling time was predicted with the lumped parameter model, and the performance of the mathematical model was checked using experimental data. It was turned out that the model could be used to predict the temperature change during the pre-cooling process of Agaricus bisporus, and it could be used to predict different conditions on pre-cooling. At the same time, the increase in cooling air velocity beyond the above values did not affect the cooling rate significantly. The pre-cooling rate was increased with the increase of the air velocity, and fitted the power function. The equation of the fitting curves between the air velocity and central temperature was obtained.