In order to meet the demands of intelligent agriculture and realize the automatic operation of rice transplanter in the field, the intelligent operation control system based on GPS navigation technique was developed. This system functionality includes automatic operation of rice transplant and automatic control of the traveling speed according to the prescription map. The YANMAR VP6 rice transplanter’s manual operation was transformed into electric control. The road experiment with GPS navigation system was accomplished on this platform. The results indicated that the electric control of the transplant operation performed accurately, the maximum average error of speed control was 0.0113m/s, and the speed control precision reached the requirement of the rice transplanter.