In order to optimize the operation parameters of the processing tomato fruit-vine separation mechanism, a rotary roller-type separation bedstand was designed. Mathematical models that described relationship between the performance indexes and influence factors of the separation mechanism were established by applying quadrate regression experiment with general rotary design method. The tendency and regularity of the separation performance influenced by the factors were analyzed. For the Ligeer 87-5 processing tomato, with a certain tomato separation rate, tomato damage rate and tomato impurity rate, the roller rotation speed was 21r/min, cam plate rotation speed was 42r/min, deflector rod oscillation amplitude was 110mm by parameter optimization.