The kinematics model was established for the rotating disc of intra-row weeding robot, and the trajectory of the rotating disc was designed by simulating its working process. Then the virtual orthogonal experiment was made in different factors and different levels. In order to optimize the best operational parameters in combination, the effects of weeding performances of the rotating disc in diverse parameters were analyzed by taking the coverage rate and the inbreaking rate as evaluation indexes. The experiment results verified that the simulation and orthogonal experiment were accurate and efficient with a good operation effectiveness of the prototype, and the weeding rate reached to 88.5%, yet the wounded seedling rate was only 1.6%. 