The pint-sized gas mixed-refrigerant liquefaction process was designed to analyze the exergy loss by using Aspen HYSYS software. The temperature and the pressure of bubble point and the dew-point were obtained from the PR equation, and the liquefied rate and energy consumption was calculated. Subsequently, the exergy loss was calculated via the thermodynamic analysis, and the methodology for reducing exergy loss was approached. An experiment was conducted to measure the exergy loss. The results showed that small gas mixed-refrigerant liquefaction process was feasible, while the largest exergy loss in the liquefaction process was compressor. With flash evaporation pre-cooled freezing medium on the top of storage tank, the reduction of exergy loss for compressor and chiller were 12.2% and 27.2%, respectively. 