25.68%提高到61.39%。In order to select suitable macroporous resin, to improve the purity of pumpkin polysaccharide and to determine purification process parameters, 9 kinds of macroporous resin were chosen to carry out static and dynamic adsorption-desorption experiments. With the indexes of adsorption capacity and elution rate, the suitable macroporous resin was selected and process parameters were confirmed. The results show that D101-I resin has the best performance of adsorption and desorption effects for purification of pumpkin polysaccharide, the optimum technology parameters are sample concentration of 4.79mg/mL, sample volume of 3.2BV, flow rate of 3BV/h, 25% ethanol for elution, elution velocity of 4BV/h, elution volume of 3.6BV. The purity of pumpkin polysaccharide increases from 25.68% to 61.39% by using the above technology. 