The synergistic lethal effect of pulsed electric fields (PEF) combined with mild heat on Listeria innocua in egg white was evaluated. The results show that 0.8, 1.4, 2.3 and 3.8 log reductions of Listeria innocua inoculated in egg white were achieved by 20, 25, 30 and 35kV/cm for 400μs at 15℃, respectively. The reductions increased to 1.3, 2.5, 4.9 and 6.4 logs when the temperature was 55℃. Approximately 1.8, 2.7, 3.5 and 3.8 log reductions of Listeria innocua were induced by 35kV/cm for 100, 200, 300 and 400μs at 15℃ respectively. The reductions increased to 2.7, 3.8, 5.4 and 6.4 logs when the temperature was 55℃. The results demonstrate the synergistic effect of PEF and mild heat on the inactivation of Listeria innocua in egg white. There is a linear relationship between inactivation of Listeria innocua and PEF applied energy.