Bone char, as an important product for the resource utilization of bone meal of different animal sources, contains rich pore structure and active sites that can be used as catalyst carriers or adsorbents in water, soil and other environments. In order to characterize the pore structure of different animal-derived bone char particles in three-dimensional and in situ visualization, porcine and bovine bone char particles with pyrolysis temperatures of 400℃, 500℃, 600℃, 700℃ and 800℃ were used. The Micro-CT scanning conditions of bone char particles were tube voltage 50kV, tube current 200mA, image resolution 10μm;Micro-CT image binarization method was Adaptive mean-C. The results showed that the Micro-CT image filtering method combining UM and ACE was optimal. The optimal parameters of the UM method were Radius value set to 5, Amount value set to 90%, Threshold value set to 0, and ACE using adaptive model. The tomographic structure images and volume model of bone char particles at different pyrolysis temperatures were similar to the electron microscopy results. The quantitative characterization results of specific surface area, porosity and pore size distribution of bone char particles were in good agreement with those determined by laboratory BET method. The results can provide a non-destructive and visualized means for in situ 3D analysis of bone char pore structure.