不同多糖条件下大豆蛋白-绿原酸凝胶特性研究
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农业农村部大豆产业技术体系岗位科学家项目(CARS-04-PS32)


Characterization of Soy Protein-Chlorogenic Acid Gels under Different Polysaccharide Conditions
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    摘要:

    为研究添加果胶、黄原胶和κ-卡拉胶3种多糖的种类和质量浓度(0.002、0.004、0.006g/mL)对大豆蛋白(SPI)-绿原酸(CA)水凝胶的凝胶特性和分子间作用力的影响,通过测定SPI-CA/多糖三元凝胶的红外光谱,分子间作用力、流变学、水分分布和微观结构等指标,最终探明不同多糖与SPI-CA相互作用机制以及不同多糖对SPI-CA凝胶性质改善的差异性和相似性。结果表明,添加黄原胶和κ-卡拉胶后,蛋白中β-折叠相对含量从24.59%升高至24.87%~26.65%,无规则卷曲含量显著降低(P<0.05),这有利于凝胶网络结构的形成。3种多糖的添加均显著增强了凝胶中氢键的作用以及凝胶中固定水的结合能力,相较于果胶和黄原胶,添加κ-卡拉胶后凝胶中的氢键的作用更强,T21的弛豫时间更短,凝胶结构也更加致密。凝胶的储能模量G′、损耗模量G″、硬度和咀嚼性均与κ-卡拉胶的浓度呈正相关。适量的果胶和黄原胶的加入能够形成稳定的凝胶,但是过度填充果胶和黄原胶会降低凝胶的热稳定性,破坏凝胶网络结构。

    Abstract:

    With the aim to investigate the effects of adding three polysaccharide types and concentrations of pectin, xanthan gum and κ-carrageenan (0.002g/mL,0.004g/mL,0.006g/mL) on the gel properties and molecular forces of soy protein (SPI)-chlorogenic acid (CA) hydrogels, the infrared spectra, molecular forces, rheology, water distribution and microstructure of SPI-CA/polysaccharide ternary gels were measured. Finally, the interaction mechanism between different polysaccharides and SPI-CA and the differences and similarities of different polysaccharides on the improvement of SPI-CA gel properties were confirmed. The results showed that the addition of xanthan gum and κ-carrageenan increased the β-sheets content of the protein from 24.59% to 24.87%~26.65% and significantly decreased the content of random coil (P<0.05), which was beneficial to the formation of the gel network structure. The addition of all three polysaccharides significantly enhanced the hydrogen bonding interactions in the gels and the binding ability of immobilized water (T21) in the gels. Compared with pectin and xanthan gum, the addition of κ-carrageenan resulted in stronger hydrogen bonding interactions in the gels, shorter relaxation time of T21, and denser gel structure. The storage modulus (G′), loss modulus (G″), hardness and chewiness of the gels were positively correlated with the concentration of κ-carrageenan. The addition of appropriate amounts of pectin and xanthan gum can form stable gels, but overfilling with pectin and xanthan gum can reduce the thermal stability of the gels and destroy the gel network structure. The results can provide theoretical support for introducing different polysaccharides to improve the gel properties of soy protein-chlorogenic acid hydrogels. 

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李杨,满慧,贾亦佳,闫馨月,闫世长,IGOR A N.不同多糖条件下大豆蛋白-绿原酸凝胶特性研究[J].农业机械学报,2023,54(5):387-395. LI Yang, MAN Hui, JIA Yijia, YAN Xinyue, YAN Shizhang, IGOR A N. Characterization of Soy Protein-Chlorogenic Acid Gels under Different Polysaccharide Conditions[J]. Transactions of the Chinese Society for Agricultural Machinery,2023,54(5):387-395.

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  • 收稿日期:2022-08-29
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  • 在线发布日期: 2023-05-10
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