In view of the unbalanced distribution of reserve resources for intensive land use, mismatch of supply and demand, deterioration of ecological environment, taking the 140 counties of Shandong Province as research units, the land use transformation and the spatiotemporal evolution characteristics of ecosystem services value were analyzed based on the remote sensing monitoring data of 2005, 2010 and 2015. Then, the improved crosssensitivity model was used to explore the impact of land use transformation on the value of ecological services, and the sensitivity partition was divided. The results showed that during the study period, the transformation of land use structure in Shandong Province was obvious, the late stage was slowed down sharply, construction land continued to be expanded, and the contradiction between land supply and demand was further intensified; the difference of regional average value of ecological services in 2005—2015 was obvious, the Shizhong District and Weishan County were highvalue areas; the Qingdao City and the Northwest Plain of Shandong Province were lowvalue areas; the transformation of farmland and waters to other land types was more sensitive to the impact of ecological service functions. The most sensitive transformation was from waters to wetlands, followed by the conversion of waters to construction land, farmland to construction land, and farmland to water. The transition of grasslands and deserts to other types of land was insensitive; ecosystem crosssensitivity had obvious regional differences. The highly sensitive areas were concentrated in spatial distribution. The highly sensitive areas of farmland, forest and construction land were concentrated in Ji’nan City and Weihai City. The highly sensitive areas of farmland and water area were concentrated in the Yellow River Delta area. The research results can provide some scientific references and decisionmaking basis for optimizing the land use pattern of Shandong Province and protecting the ecological environment. 