As important water source of the Middle Route Project of the SouthtoNorth Water Diversion in China, the protection and construction of ecological environment in water supply area is top priority of sustainable development of ecological environment in China. With the implementation of water diversion project, the land use and cover situation in Danjiang River Basin would be inevitably changed. Based on data source of 16 phases of Landsat TM/OLI and HJ-1A CCD image in 2002—2017 in the study area, the land use change map was extracted, the land use/cover change (LUCC) trajectory analysis method of improved stable mapping method was established, and the relationship between similarity, turnover and diversity (STD) index of stable mapping and the number of time phases was derived; the change track was divided into five first level track processes: stable, gradual, discontinuous gradual, cyclic and fluctuating, and further subdivided into two and three level track processes. On this basis, combined with quantitative models such as land use dynamic degree and landscape index, the overall characteristics and spatiotemporal changes of the study area were analyzed. The results showed that: during 2002—2017, the cultivated land in the study area was decreased significantly, the construction land and water area continued to increase, the forest land and grassland were firstly decreased and then increased, and the bare land was relatively stable; the main flow of land use change in the study area was concentrated in the construction land and water area, which was transformed from cultivated land; at the same time, due to the construction of Danjiangkou reservoir area and the implementation of the Middle Route Project of South to North Water Diversion, the water area was increased from 218.60km2 to 400.31km2 in length. Affected by the natural topography, the woodland in the northern mountainous and hilly areas of the study area was well protected, while the woodland, cultivated land and grassland in the transition zone between hills and plains had significantly transformed each other, accounting for 5.85% of the total area of the study area. The results can provide data support and decisionmaking basis for the sustainable utilization of land resources, protection of ecological environment and protection of water sources. 