李杨,钟明明,廖一,谢凤英,孙禹凡,齐宝坤.超高压下大豆亲脂蛋白-羟丙基甲基纤维素理化性质研究[J].农业机械学报,2019,50(12):365-371.
LI Yang,ZHONG Mingming,LIAO Yi,XIE Fengying,SUN Yufan,QI Baokun.Physicochemical Properties of Soy Lipophilic Protein-Hydroxypropyl Methylcellulose Complex under Ultra-high Pressure[J].Transactions of the Chinese Society for Agricultural Machinery,2019,50(12):365-371.
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超高压下大豆亲脂蛋白-羟丙基甲基纤维素理化性质研究   [下载全文]
Physicochemical Properties of Soy Lipophilic Protein-Hydroxypropyl Methylcellulose Complex under Ultra-high Pressure   [Download Pdf][in English]
投稿时间:2019-04-07  
DOI:10.6041/j.issn.1000-1298.2019.12.042
中文关键词:  大豆亲脂蛋白  羟丙基甲基纤维素  复合物  超高压
基金项目:中国博士后科学基金面上项目(2018M631902)和国家自然科学基金重点项目(31430067)
作者单位
李杨 东北农业大学 
钟明明 东北农业大学 
廖一 东北农业大学 
谢凤英 东北农业大学 
孙禹凡 东北农业大学 
齐宝坤 东北农业大学 
中文摘要:大豆亲脂蛋白(Lipophilic protein, LP)和羟丙基甲基纤维素(Hydroxypropyl methylcellulose, HPMC)在中性条件下(pH值74)复合后,可自发组成LP-HPMC复合物,但仍有部分未自组装的LP和HPMC存在于溶液中,目前仍无法实现LP-HPMC最大程度复合以及最多结合位点的目标。采用荧光光谱和红外光谱研究了不同超高压处理对LP-HPMC复合程度以及作用力的影响,采用乳化活性和乳化稳定性测定、表面疏水性分析、动态光散射粒度分析等解析了超高压对LP-HPMC结构变化与功能性质表达的构效关系。结果表明:LP-HPMC复合物通过非共价相互作用结合,蛋白二级结构发生相应改变。当压力为400MPa时,复合物有最大的溶解度和最小的表面疏水值,分别为41.1%和57.832,此时乳化活性和乳化稳定性指标也最佳。
LI Yang  ZHONG Mingming  LIAO Yi  XIE Fengying  SUN Yufan  QI Baokun
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:soy lipophilic protein  hydroxypropyl methyl cellulose  complex  ultra high pressure
Abstract:Soy lipophilic protein (LP) and hydroxypropyl methylcellulose (HPMC) can form an LP-HPMC complex spontaneously after being combined under neutral conditions (pH value 7.4), but there are still some self assembled LP and HPMC presented in solution. At present, the research still can not achieve the maximum compounding degree of LP-HPMC and the target of the most binding sites. Fluorescence and infrared spectroscopy were used to study the effects of different ultra high pressure treatments on the degree of LP-HPMC compounding and the force. The effects of ultra high pressure on structure activity relationship of structural changes and functional properties of LP-HPMC were analyzed by emulsion activity and emulsion stability measurement, surface hydrophobicity analysis and dynamic light scattering particle size analysis. The results showed that the LP-HPMC complex was bound by non covalent interaction and the secondary structure of the protein was changed accordingly. When the pressure was 400MPa, the maximum solubility and minimum surface hydrophobic value of the composite were 41.1% and 57.832, respectively, and the emulsification activity and emulsion stability index were also the best.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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