基于高光谱成像的生鲜鸡肉糜中大豆蛋白含量检测
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国家自然科学基金面上项目(31772062)和国家重点研发计划项目(2018YFC1603500)


Detection of Soybean Protein Content in Fresh Minced Chicken Meat Using Hyperspectral Imaging
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    摘要:

    为快速无损检测生鲜鸡肉糜中超量添加的大豆蛋白含量,采集了鸡肉糜中掺杂的3种典型大豆蛋白的高光谱图像。在鸡肉糜中分别掺入质量分数0~30%的大豆蛋白粉(SPF)、大豆浓缩蛋白(SPC)和大豆分离蛋白(SPI),并于可见-近红外(400~1000nm)光谱范围采集样品的高光谱图像,基于波段运算提取感兴趣区域的平均光谱,建立了原始及预处理光谱的偏最小二乘回归(PLSR)定量模型,发现模型对SPC预测效果最优(R2p=0.9984)、SPF次之、SPI最差。进一步利用二维相关光谱(2DCOS)自相关峰提取特征波长,建立的多光谱模型对于3种大豆蛋白的检测限分别可达0.53%、0.58%和1.02%。将以上多光谱模型应用到原始光谱图像,实现了不同大豆蛋白及其掺假梯度的可视化表征。

    Abstract:

    In order to rapidly and nondestructively detect the excessive soybean protein content in fresh minced chicken meat, hyperspectral images of three typical soybean protein adulterated in minced chicken meat were collected. Soybean protein flour (SPF), soybean protein concentrate (SPC) and soybean protein isolates (SPI) with 0~30% adulteration levels were mixed into the minced chicken meat. The hyperspectral images of prepared samples were collected in the visible and near infrared (400~1000nm) spectral range. The average spectra of regions of interest were extracted based on band operation and models based on the original and preprocessed spectra were established. Partial least squares regression (PLSR) quantitative models showed that the optimal performance were best for SPC (R2p=0.9984), followed by SPF, and then SPI. The twodimensional correlation spectrum (2DCOS) was used to extract the characteristic wavelengths to further establish multispectral models. The limits of detection (LODs) of the multispectral models were found to be 0.53%, 0.58% and 1.02% for SPF, SPC and SPI, respectively. The multispectral models above were also applied back to the raw multispectral images to visualize the distribution of different soybean proteins, as well as their adulteration levels. Results showed that the distribution differences within samples and among samples could be well visualized.

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王伟,姜洪喆,贾贝贝,鹿瑶.基于高光谱成像的生鲜鸡肉糜中大豆蛋白含量检测[J].农业机械学报,2019,50(12):357-364.

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  • 收稿日期:2019-07-24
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  • 在线发布日期: 2019-12-10
  • 出版日期: 2019-12-10