During the kiwifruit flower pollination, depositing sufficient pollen on the kiwifruit flower stigma is the key to ensure the yield and quality of kiwifruit. To improve kiwifruit pollination quality and reduce assumption of expensive pollen, kiwifruit flower pollination based on air-liquid nozzle spraying was studied. By means of an air-liquid pollination device, using the methods of droplets sedimentation weighing and image grayscale analysis, the effects of spray pressure, pollen suspension flow rate and spraying distance on the spray quality and distribution characteristics of pollination suspension were investigated to get optimum control parameters of spraying air pressure, pollen suspension flow rate and distance between nozzle and kiwifruit flower. Moreover, these control parameters were validated through kiwifruit flower pollination experiment on the scaffolding kiwifruit orchard. The test result showed that the air-liquid spray pollination method under these spray parameters can effectively improve the kiwifruit setting rate, the single kiwifruit weight and reduce kiwifruit deformity rate. The experiments showed that when applying the pollen liquid with mass fraction of 0.1% at petal fully opening stage, a single kiwifruit flower needed at least 42.9mg effective deposition of pollen liquid in the stigma area to pollinate all stigmas. Under the air pressure of 0.20MPa, the pollen liquid flow of 0.125L/min, the spray pulse duration of 0.1s and the spray distance of 35cm, the air-liquid spraying pollination can notably improve the effective deposition of pollen liquid on the stigma and the quality of the pollination. Pollination test on kiwifruit orchard showed that the kiwifruit setting rate and average kiwifruit weight as a pollination result of the air-liquid spraying pollination was 86.7% and 91.5g respectively, which was 13.4 percent points and 8.0g higher than that of the electric sprayer, respectively.