Effects of ultrasonic treatments with different powers (200W, 400W) for different durations (6min, 12min and 24min) on the stability of recombinant oil emulsions were investigated. Functional properties of the composite system as a function of ultrasonic conditions were explored at the same time. Circular dichroism spectrum, particle size distribution, ζ-potential, FI and emulsibility were determined. The emulsifying properties of the reconstituted oil body emulsion was significantly increased. At the same time, the volume average particle size was decreased from 1717nm to 337.5nm, the absolute value of ζ-potential was increased, and the α-helix content was decreased to 10.4%. And the solution had homogeneous distribution and stable properties. However, with the further increase of ultrasonic power, the surface protein of the recombinant oil body was insoluble, and the functional properties of the recombinant emulsion was decreased. This indicated that the ultrasonic treatment would affect the stability of the recombinant emulsion, and the ultrasonic treatment of suitable strength was beneficial to the formation of the recombinant oil emulsion.