王琳琳,陈炼红,韩玲,李键,余群力,蔡自建.茶多酚对宰后牦牛肉线粒体细胞凋亡和肌肉嫩度的影响[J].农业机械学报,2019,50(10):352-359.
WANG Linlin,CHEN Lianhong,HAN Ling,LI Jian,YU Qunli,CAI Zijian.Effects of Tea Polyphenols on Mitochondrial Apoptosis and Meat Tenderness in Post mortem Yak Meat[J].Transactions of the Chinese Society for Agricultural Machinery,2019,50(10):352-359.
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茶多酚对宰后牦牛肉线粒体细胞凋亡和肌肉嫩度的影响   [下载全文]
Effects of Tea Polyphenols on Mitochondrial Apoptosis and Meat Tenderness in Post mortem Yak Meat   [Download Pdf][in English]
投稿时间:2019-07-12  
DOI:10.6041/j.issn.1000-1298.2019.10.041
中文关键词:  牦牛肉  茶多酚  氧化应激  细胞凋亡  肌肉嫩度
基金项目:中央高校基本科研业务费专项资金项目(2019NQN43)、国家自然科学基金项目(31560463)和国家肉牛牦牛产业技术体系项目(CARS-38)
作者单位
王琳琳 西南民族大学 
陈炼红 西南民族大学 
韩玲 甘肃农业大学 
李键 西南民族大学 
余群力 甘肃农业大学 
蔡自建 西南民族大学 
中文摘要:为探究茶多酚对宰后牦牛肉成熟过程中线粒体氧化应激损伤介导的线粒体细胞凋亡级联反应及对肌肉嫩度的影响,以经茶多酚处理的牦牛背最长肌为研究对象,测定对照组和茶多酚处理组线粒体氧化应激水平、线粒体氧化损伤程度、线粒体功能特性、线粒体细胞凋亡进程,以及肌肉嫩度的变化。结果表明,在宰后成熟早中期,茶多酚组线粒体活性氧(Reactive oxygen species,ROS)水平、丙二醛(Malondialdehyde,MDA)以及羰基含量整体上显著或极显著低于对照组;成熟中后期,线粒体超氧化物歧化酶(Superoxide dismutase,SOD)活性以及线粒体膜流动性显著或极显著高于对照组。24h后,茶多酚组线粒体通透性转换孔(Mitochondrial permeability transition pore,MPTP)开放程度显著或极显著低于对照组;6~168h,茶多酚组线粒体Cyt c浓度均高于对照组;72~168h,胞浆细胞色素c(Cytochrome c,Cyt c)浓度均显著或极显著高于对照组;在成熟早期,细胞凋亡酶 3(Caspase 3)活性以及肌原纤维小片化指数(Myofibrillar fragmentation index,MFI)显著或极显著低于对照组。以上研究结果说明,茶多酚通过抑制ROS介导的氧化应激对线粒体结构和功能的损伤,起到抑制线粒体细胞凋亡级联反应对肌肉嫩度的改善作用,表明茶多酚在发挥抗氧化剂和保鲜剂作用、提高肌肉品质的同时,从细胞凋亡嫩化机制角度出发,对肌肉的嫩化产生不利影响。
WANG Linlin  CHEN Lianhong  HAN Ling  LI Jian  YU Qunli  CAI Zijian
Southwest University for Nationalities,Southwest University for Nationalities,Gansu Agricultural University,Southwest University for Nationalities,Gansu Agricultural University and Southwest University for Nationalities
Key Words:yak meat  tea polyphenols  oxidative stress  apoptosis  meat tenderness
Abstract:Aiming to investigate the effects of tea polyphenols on the mitochondrial apoptosis cascade reaction mediated by mitochondrial oxidative stress and tenderness of yak meat during postmortem aging. The longissimus dorsi muscles injected with inhibitor tea polyphenol were taken as the experiment objects. The levels of mitochondrial oxidative stress, degree of mitochondrial oxidative damage, mitochondrial functional characteristics, process of mitochondrial apoptosis and change of meat tenderness in the control group and the treatment group were measured. The results showed that the level of mitochondrial ROS, MDA and carbonyl content in the treatment group was overall significantly or extremely significantly lower than that in the control group in the early of postmortem aging. At the middle and late of aging time, the SOD activity and the mitochondrial membrane fluidity was significantly or extremely significantly higher than that in the control group. After 24h, opening of the MPTP in the treatment group was significantly or extremely significantly lower than that in the control group. After 6~168h, the Cyt c concentration of mitochondria in the treatment group was higher than that in the control group; during 72~168h, the Cyt c concentration of cytoplasm in the treatment group was significantly or extremely significantly higher than that in the control group. Meanwhile, Caspase 3 activity and MFI in the treatment group was significantly or very significantly lower than that in the control group at early aging time. The research demonstrated that tea polyphenols inhibited the role of the mitochondrial apoptosis cascade reaction on the meat tenderization by inhibiting ROS mediated oxidative stress damage of mitochondrial structure and function. These observations indicated that tea polyphenols acted as the antioxidants and antistaling agent in improving the quality of meat at the same time, may have adverse effects on meat tenderization from the angle of the mechanism of apoptosis tenderization and further confirmation was needed.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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