With the development of space technology, the highprecision instruments carried by spacecraft have higher requirements on the vibration environment. Active control of spacecraft microvibration was studied and analyzed. Firstly, according to the different vibration isolation requirements, the openloop transfer function of disturbance displacement transmission and disturbance force transmission of singleaxis vibration isolator and the closedloop transfer function based on force feedback were established respectively. Then, the disturbance displacement suppression was taken as an example to theoretically derive. The active vibration isolation effect based on force feedback PI control and linear active disturbance rejection control was analyzed, and the influence of Butterworth filter on active vibration isolation system was discussed. Finally, a singleaxis active vibration isolation experimental platform was built to verify the correctness of the theoretical derivation results. Linear active disturbance rejection control can achieve better vibration isolation effect in the full frequency range of 20~500Hz. The attenuation rate of microvibration can reach 10dB in the low frequency band and 20dB in the resonant frequency and high frequency.