Based on comprehensive consideration of the comprehensive quality of cultivated land, autocorrelation and landscape patterns, the demarcation of permanent basic farmland is of great significance to regional food security and ecological security. Taking Chongren County as an example, GIS platform and GeoDa software were used to evaluate the comprehensive quality of cultivated land through the construction of comprehensive quality evaluation system of cultivated land, and spatial autocorrelation analysis was carried out according to the results of comprehensive quality assessment, and then according to the results of autocorrelation analysis and the ecological security of cultivated land landscape and correspondence between patterns to describe permanent basic farmland. The soil physical and chemical properties, farming conditions were comprehensively considered into the research, and arable land conditions in the study area, and a permanent basic farmland delineation method was proposed based on the comprehensive quality of farmland, comprehensive quality cluster relationship and cultivated land landscape structure, and delineated permanent basic farmland in Chongren County as 305.94km2, the ploughed land was more in line with the requirements of the permanent basic farmland, which would contribute to the longterm management and protection of basic farmland, and also provide a reference for the comprehensive quality evaluation of farmland and the demarcation of basic farmland.