Separating carrot tassel from fruit by pulling can meet the requirement of tassel fruit separation better. In order to provide design parameters for pull type carrot tassel fruit separation device, and obtain the best separation effect, the influence of related parameters on the pull rod kinematics characteristics should be analyzed qualitatively. To analyze the influence of relevant parameters on the kinematic characteristics of pull rod accurately, the kinematics model of pulling device type tassel fruit separation should be established. To verify the correctness of the previous theoretical analysis, the pull type carrot tassel fruit separation test device was designed. Then a series of orthogonal tests were carried out to explore the factors that affecting the effect of tassel fruit separation. In the orthogonal testing, the pull rod speed, belt line speed and the angle between pull rod and conveyer belt were test factors. The comparison between the results of test and theoretical analysis showed that the pull rod speed had the biggest influence on carrot tassel fruit separation effect, and angle between pull rod and conveyer belt also had significant effects on carrot tassel fruit separation effect. At the same time, the best combination of parameters in carrot tassel fruit separating device was obtained as follows: pull rod speed was 200r/min, belt line speed was 1.2m/s, and angle between pull rod and conveyer belt was 40°. The results showed that the concordance rate in the tassel fruit separation was 97%, the success rate was 94%, and the injury rate was 7.7%.