李杨,胡淼,谢凤英,齐宝坤,孙禹凡.超高压均质对紫苏油纳米乳液稳定性的影响[J].农业机械学报,2018,49(8):381-387.
LI Yang,HU Miao,XIE Fengying,QI Baokun,SUN Yufan.Effect of Ultrahigh Pressure Homogenization on Stability of Perilla Oil Nanoemulsion[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(8):381-387.
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超高压均质对紫苏油纳米乳液稳定性的影响   [下载全文]
Effect of Ultrahigh Pressure Homogenization on Stability of Perilla Oil Nanoemulsion   [Download Pdf][in English]
投稿时间:2018-04-12  
DOI:10.6041/j.issn.1000-1298.2018.08.046
中文关键词:  紫苏油  纳米乳液  超高压均质  稳定性
基金项目:国家自然科学基金面上项目(31571876)、霍英东教育基金会高等院校青年教师基金项目(20152325210002)和中国博士后科学基金面上项目(2017M612315)
作者单位
李杨 东北农业大学 
胡淼 东北农业大学 
谢凤英 东北农业大学 
齐宝坤 东北农业大学 
孙禹凡 东北农业大学 
中文摘要:采用大豆分离蛋白(Soybean protein isolate, SPI)-磷脂酰胆碱作为表面活性剂,利用超高压均质技术制备紫苏油纳米乳液,探究不同SPI质量分数以及超高压均质压力对紫苏油纳米乳液稳定性的影响。结果表明:随着SPI质量分数和超高压均质压力的增加,紫苏油纳米乳液的平均粒径和PDI值逐渐降低,在SPI质量分数为4%、均质压力140MPa条件下紫苏油纳米乳液均一稳定,粒径最小为241.03nm,PDI值最低为0.13;经过21d的储藏试验,用Turbiscan稳定性分析仪测定紫苏油纳米乳液的物理稳定性,在SPI质量分数4%、均质压力140MPa条件下,紫苏油纳米乳液未发生脂肪上浮或溶液聚集等现象,同时纳米乳液的平均粒径也未发生较大的变化,表明紫苏油纳米乳液在这一条件下具有较强的储藏稳定性。通过圆二色谱测定紫苏油纳米乳液中界面蛋白的二级结构变化,结果表明,超高压均质使α-螺旋的相对含量降低,而无规卷曲和β-折叠的相对含量升高,这说明界面蛋白的二级结构的变化对紫苏油纳米乳液的稳定性有一定的影响。
LI Yang  HU Miao  XIE Fengying  QI Baokun  SUN Yufan
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:perilla oil  nanoemulsions  ultrahigh pressure homogenization  stability
Abstract:Perilla oil nanoemulsions, stabilized soybean protein isolated protein (SPI) -phosphatidyl choline, was prepared by using ultrahigh pressure homogenous technology. The effects of different SPI concentrations and ultrahigh homogenous pressures on stability of perilla oil nanoemulisons were researched. The results showed that with the increase of SPI concentration and ultrahigh homogenous pressure, the mean particle size and PDI were gradually decreased. The perilla oil nanoemulsions had the smallest mean particle size (230nm) and the lowest PDI value (0.15) under the condition of 4% SPI concentration and 140MPa ultrahigh homogenous pressure, which showed that the perilla oil nanoemulsions was homogeneous under this condition. After storage experiment for 21d, perilla oil nanoemulsions had strong physical stability by using Turbiscan stability analyzer and no change of mean particle size at 4% SPI concentration and 140MPa ultrahigh homogenous pressure. There was no fat separation or aggregation in the nanoemulsions with 4% SPI concentration and 140MPa ultrahigh homogenous pressure. The content of α-helix was decreased gradually but the content of β-fold and random coil were increased by using circular dichroism spectrum, which demonstrated that the change of the secondary structure of interfacial protein had a certain effect on the stability of perilla oil nanoemulsion. Study on the stability of perilla oil nanoemulsion provided a theoretical basis for the development of novel nano foods and the industrialized production of nanoemulsions. 

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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