江连洲,佟晓红,刘宝华,王欢,张巧智,李杨.酶种类对生物解离大豆蛋白酶解物功能性和苦味的影响[J].农业机械学报,2018,49(8):368-374.
JIANG Lianzhou,TONG Xiaohong,LIU Baohua,WANG Huan,ZHANG Qiaozhi,LI Yang.Effect of Enzymatic Species on Function and Bitterness of Enzyme-assisted Aqueous Extraction Soybean Protein Hydrolysates[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(8):368-374.
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酶种类对生物解离大豆蛋白酶解物功能性和苦味的影响   [下载全文]
Effect of Enzymatic Species on Function and Bitterness of Enzyme-assisted Aqueous Extraction Soybean Protein Hydrolysates   [Download Pdf][in English]
投稿时间:2018-02-06  
DOI:10.6041/j.issn.1000-1298.2018.08.044
中文关键词:  蛋白酶解物  功能性  苦味  生物解离  傅里叶红外光谱
基金项目:国家重点研发计划项目(2016YFD0401402)和中国博士后科学基金特别项目(2017T100507)
作者单位
江连洲 东北农业大学 
佟晓红 东北农业大学 
刘宝华 东北农业大学 
王欢 东北农业大学 
张巧智 东北农业大学 
李杨 东北农业大学 
中文摘要:在生物解离提取大豆油脂过程中会伴随产生丰富的水解液,水解液主要成分为大豆蛋白酶解物。以不同种类蛋白酶(碱性蛋白酶、中性蛋白酶、木瓜蛋白酶、菠萝蛋白酶、风味蛋白酶、复合蛋白酶和胰蛋白酶)对挤压膨化大豆粉进行酶解,将得到的不同蛋白酶解物作为研究对象,采用水解度、SDS-PAGE凝胶电泳、氨基酸组成分析、傅里叶红外光谱对大豆蛋白酶解物结构进行表征,利用乳化活性和乳化稳定性、溶解性及感官评价等表征大豆蛋白酶解物功能性和苦味。结果显示:采用碱性蛋白酶进行生物解离时,提油率最高,为91.23%;碱性蛋白酶解物溶解性为91.12%,但是酶解产物的苦味值大大增加,乳化性也不理想。采用风味蛋白酶处理时,尽管提油率不是最高,为87.73%,但是酶解产物苦味值为1.5,溶解性为87.12%,乳化活性和乳化稳定性效果最优。
JIANG Lianzhou  TONG Xiaohong  LIU Baohua  WANG Huan  ZHANG Qiaozhi  LI Yang
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:protein hydrolysates  function  bitterness  enzyme-assisted aqueous extraction  Fourier transform infrared spectroscopy
Abstract:Enzyme-assisted aqueous extraction process is a green oil extraction technology,which has advanced in recent years, may be a effective alternative technology to separate oil and protein from soybeans. It will be accompanied by cream, skim and residual fraction in the enzyme assisted aqueous extraction process.The main component in the skim is soy protein hydrolysates. Different types of proteases (Alcalase, Neutrase, Papain, Bromelain, Flavourzyme, Protamex and Trypsin) were used to hydrolyze the extruded soybean flour. Different kinds of protein hydrolysates were used as the research objects. The structure of soybean protein hydrolysates was characterized by degree of hydrolysis, SDS-polyacrylamide gel electrophoresis, amino acid composition analysis and Fourier transform infrared spectroscopy. Solubility, emulsion activity index and emulsion stability index characterized the functional of the soy protein hydrolysates. The bitterness value of the soy protein hydrolysates was obtained by sensory evaluation. The results indicated that when using Alcalase for enzyme assisted aqueous extraction, the highest oil extraction rate was 91.23% and the solubility of soybean protein hydrolyzate was 91.12%. However, the bitterness value of the hydrolyzate was increased greatly which was 4, the emulsion activity index and emulsion stability index were not good. When using Flavourzyme for enzyme assisted aqueous extraction treatment, the oil extraction was 87.73% and the solubility was 87.12%. However, the bitterness of the hydrolyzates was only 1.5, thus the sensory evaluation was the best. And the hydrolyzates had the best emulsion activity index and emulsion stability index.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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