王欢,佟晓红,钟明明,齐宝坤,李杨,江连洲.微波干燥对生物解离富肽豆粉蛋白亚基及功能性的影响[J].农业机械学报,2018,49(7):363-368.
WANG Huan,TONG Xiaohong,ZHONG Mingming,QI Baokun,LI Yang,JIANG Lianzhou.Effect of Microwave Drying on Protein Subunits and Functional Properties of Enzyme-assisted Aqueous Extraction Processing Protein Rich Bean Powder[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(7):363-368.
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微波干燥对生物解离富肽豆粉蛋白亚基及功能性的影响   [下载全文]
Effect of Microwave Drying on Protein Subunits and Functional Properties of Enzyme-assisted Aqueous Extraction Processing Protein Rich Bean Powder   [Download Pdf][in English]
投稿时间:2018-01-23  
DOI:10.6041/j.issn.1000-1298.2018.07.044
中文关键词:  富肽豆粉  微波干燥  生物解离  高效液相体积排阻色谱
基金项目:国家重点研发计划项目(2016YFD0401402)和霍英东教育基金青年教师基金项目(151032)
作者单位
王欢 东北农业大学 
佟晓红 东北农业大学 
钟明明 东北农业大学 
齐宝坤 东北农业大学 
李杨 东北农业大学
哈尔滨市食品产业研究院 
江连洲 东北农业大学 
中文摘要:以生物解离大豆水解液为研究对象,运用高效液相体积排阻色谱技术研究了不同微波干燥条件对富肽豆粉中蛋白类物质亚基组分和空间构象的变化规律,并对生物解离富肽豆粉的粉体性质及功能性质进行研究。结果表明,微波干燥条件对HPLC-SEC峰的强度影响明显,分子量18~44ku的蛋白质水解物约占总蛋白质类的73.47%~89.36%,主要亚基成分为大豆球蛋白,这是由于不同微波干燥条件下的物相转换率和分子间碰撞机会不同,蛋白纤维化聚集形成的速度不同,从而对生物解离富肽豆粉分子量产生影响。此外,生物解离富肽豆粉的水溶性和分散性在不同微波干燥条件下具有相似趋势,这与粉体残余水分量、蛋白质分子运动剧烈程度、分子构象变化及分子聚集和解离相关。
WANG Huan  TONG Xiaohong  ZHONG Mingming  QI Baokun  LI Yang  JIANG Lianzhou
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University;Harbin Food Industry Research Institute and Northeast Agricultural University
Key Words:protein peptide-rich bean powder  spray-microwave drying  enzyme-assisted aqueous extraction  HPLC-SEC
Abstract:Biological dissociated soybean hydrolysates, which was yield in such of the enzyme assisted aqueous extraction of soybean oil processing, was used as research object, and applied for spray and microwave drying of peptide rich soybean protein meal powder, which was prepared under different conditions. The protein subunits of soybean meal composition, the rules of spatial conformation change of the protein phase volume, and the physical and functional properties of the peptide-rich soybean meal powder were in-depth investigated based on high performance liquid exclusion chromatography technology. Research results showed that the impact of spray microwave drying conditions on the HPLC-SEC peak intensity was significant, and 18~44ku proteins took 73.47%~89.36% of the total protein of the powder. The main component of soybean protein was globulin subunit, which was perhaps determined by the phase conversion rate, molecular collision between different proteins, aggregation opportunities and fibrosis at different spray speeds and microwave drying conditions, molecular weight of the enzyme dissociated soybean peptide-rich powder were also influenced under various conditions. In addition, the water solubility and dispersibility of biodissociated peptide-rich soy flour had similar trends under different microwave drying conditions, and reflected the same problem, which were related to the residual water content of the powder, the intensity of protein molecule movement, conformational changes, and aggregation and dissociation.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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