The technique of planting on film can effectively prevent soil and water loss, which is beneficial to the planting of organic rice. The pot seedling transplanting has the advantages of non-hurting root and no need of recovering period, so it can extend the crop growing period, increase and bring forward the effective tillering, which is very important to the food security. In order to realize the combination of two kinds of agronomy, a new type of transplanting mechanism was designed, in which the hole was perforated on film and the rice pot seedling was planted, it was effective to avoid the damage of seedling caused by the unsynchronization of film breaking and rice planting. The working principle of rice pot seedling transplanting mechanism was introduced, kinematic analysis model of mechanism was established, a computer aided analysis and optimization software of the transplanting mechanism was developed, and a set of structural parameters was obtained to meet the requirement of transplanting on film. Three-dimensional model was built, and input to ADAMS software to conduct virtual kinematic simulation analysis. The physical prototype of the transplanting mechanism was developed to conduct kinematic tests with high-speed photography technology on the test-bed. The result of bench test was basically consistent with the track obtained from the theoretical analysis, which showed the correctness of the design of transplanting mechanism. The seedling picking and the planting on film were completed on the test bench, the success rate of seedling picking-up was 92.8%, and the qualification rate of transplanting on film was 90%, which met the operation requirement of transplanting on film and showed the feasibility of performance of transplanting mechanism of rice pot seedling on film. The study provided useful reference for the related design and had an important significance.