王殿龙,吴波,艾平,辛娅,何明雄,张衍林.两步预处理对厌氧消化产酸纤维酶解和沼气发酵的影响[J].农业机械学报,2018,49(3):322-327.
WANG Dianlong,WU Bo,AI Ping,XIN Ya,HE Mingxiong,ZHANG Yanlin.Effect of Two-step Pretreatment on Enzymatic Hydrolysis and Biogas Fermentation of Anaerobically Digested Fiber from VFAs Production[J].Transactions of the Chinese Society for Agricultural Machinery,2018,49(3):322-327.
摘要点击次数: 1823
全文下载次数: 985
两步预处理对厌氧消化产酸纤维酶解和沼气发酵的影响   [下载全文]
Effect of Two-step Pretreatment on Enzymatic Hydrolysis and Biogas Fermentation of Anaerobically Digested Fiber from VFAs Production   [Download Pdf][in English]
投稿时间:2017-08-08  
DOI:10.6041/j.issn.1000-1298.2018.03.039
中文关键词:  厌氧消化产酸纤维  酶解  沼气发酵  两步预处理
基金项目:国家自然科学基金项目(51406064)、中国农业科学院基本科研业务费前沿探索项目(Y2017JC39)、四川省科技支撑计划项目(17ZDYF1305)和农业部沼气科学研究所基本科研业务费项目(1610012016020)
作者单位
王殿龙 淮阴工学院 
吴波 农业部沼气科学研究所 
艾平 华中农业大学 
辛娅 淮阴工学院 
何明雄 农业部沼气科学研究所 
张衍林 华中农业大学 
中文摘要:对厌氧消化产酸纤维(挥发性脂肪酸生产废渣)进行了酶解糖化和沼气发酵实验研究,探索了乙酸氨水两步预处理对木质纤维素降解、葡萄糖浓度、木糖浓度和沼气产率的影响。结果表明:乙酸氨水两步预处理进一步降解了厌氧消化产酸中的半纤维素和木质素,而且纤维素回收率较高,为77.47%~81.44%,其中4%乙酸处理和10%氨水处理实验组的纤维素质量分数达到56.82%,半纤维素质量分数仅为7.94%;在上述预处理条件下,酶解糖化效率最高,葡萄糖质量浓度达到47.46g/L,葡萄糖得率为67.73%;同时在此乙酸氨水两步预处理条件下的沼气产率明显优于单独乙酸和单独氨水预处理,获得的最高沼气产率为414mL/g,有效提高了厌氧消化产酸纤维的产气效率。
WANG Dianlong  WU Bo  AI Ping  XIN Ya  HE Mingxiong  ZHANG Yanlin
Huaiyin Institute of Technology,Biogas Institute of Ministry of Agriculture,Huazhong Agricultural University,Huaiyin Institute of Technology,Biogas Institute of Ministry of Agriculture and Huazhong Agricultural University
Key Words:anaerobically digested fiber from VFAs production  enzymatic hydrolysis  biogas fermentation  two-step pretreatment
Abstract:The enzymatic hydrolysis and biogas fermentation of anaerobically digested fiber from volatile fatty acids (VFAs) production with rice straw were studied. The effects of two-step pretreatment of acetic acid and aqueous ammonia on the degradation of lignocellulose, glucose concentration, xylose concentration and biogas production were explored. The results showed that two-step pretreatment of acetic acid and aqueous ammonia can further degrade the hemicellulose and lignin in anaerobically digested fiber from VFAs production, and the recovery rate of cellulose was higher, which was 77.47%~81.44%. Among them, the mass fraction of cellulose was 56.82% and the mass fraction of hemicellulose was only 7.94% for pretreatment of 4% acetic acid and 10% aqueous ammonia at 121℃. Under the above pretreatment conditions, the enzymatic saccharification efficiency was the highest, the mass concentration of glucose was 47.46g/L, and the glucose yield was 67.73%. Furthermore, the biogas production rate of anaerobically digested fiber from volatile fatty acids production pretreated by two-step pretreatment was better than those of acetic acid pretreatment alone and aqueous ammonia pretreatment alone. The highest biogas yield (414mL/g) was obtained. Two-step pretreatment effectively improved the biogas yield of anaerobically digested fiber from VFAs production. Therefore, the research proved a new biorefinery way of anaerobically digested fiber.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

   下载PDF阅读器