In order to investigate the effect of rhizosphere aeration on NH+4-N and NO-3-N absorption and metabolism of red globe grape seedlings, the experiment was done by taking 2-year old red globe grape seedling as test material, through subsurface drip irrigation（SDI） with tanks technique and 15N tracer technique to study effect of the rhizosphere aeration treatment on plant growth and NH+4-N and NO-3-N absorption, distribution and utilization. The results showed that rhizosphere aeration under SDI with tanks could promote the growth of new shoots, increase the stem diameter, and especially significantly increase the dry matter accumulation of new shoots and fine roots. In the same irrigation period, rhizosphere aeration under SDI with tanks increased the SPAD value and net photosynthetic rate of the leaves, but it had negative effects on the instantaneous water use efficiency of the plant leaves. The nitrogen isotope tracer results showed that grape new organs on the NO-3-N Ritchie ability, absorption and utilization efficiency were significantly higher than that of NH+4-N under aeration treatment and without aeration treatment, and the results showed that rhizosphere aeration under SDI with tanks did not affect the nitrate preference of grape plants. The results also showed that gas injection treatment can significantly promote the absorption of nitrate uptake and utilization of new organs, inhibition of ammonium nitrogen, which suggested that nitrate nitrogen should be selected as nitrogen source under the condition of rhizosphere aeration under SDI with tanks.