In order to clarify the distribution characteristics of dissolved oxygen in water in symbiosis system, the water quality parameters, meteorological data and biomass information of the system were extracted by using the multi-point sampling device and the networking sensor system. The influence of light intensity and feed intake on the spatiotemporal variation of dissolved oxygen in the symbiosis system was studied by correlation analysis method. On the one hand, the results showed that dissolved oxygen levels decreased with the increase of vertical depth in the fish pond, the correlation was between -0.9 and -0.7. The dissolved oxygen was changed at 9:00, and there was obvious correlation between the logarithm and vertical depth of dissolved oxygen with correlation coefficient value r=0.9894 and the value of the variance analysis F=126.94. On the other hand, the dissolved oxygen decreased with the horizontal increase of the water culture tank following the correlation coefficient from -0.9 to -0.8. A significant linear relationship reached 0.89 between the dissolved oxygen content and the horizontal distance. In addition, there was the logarithmic relationship between the dissolved oxygen content of hydroponic tank and light intensity, in which the correlation coefficient was 0.8158. According to the monitoring during different time, the diurnal variation of dissolved oxygen was affected by the spatial variation. The fluctuation values of dissolved oxygen content decreased during the daytime and increased during the nighttime which might be mainly related to the change of light intensity, feeding operation and consumption. As a result, the study provides a theoretical basis for the study of dissolved oxygen in the symbiosis system.