Bacillus amyloliquefaciens is a kind of plant growth promoting rhizobacteria (PGPR). This kind of beneficialrhizosphere bacterium plays a key role in increasing soil fertility, promoting plant growth and suppressing the growth of phytopathogens in the field of agriculture. This characteristics of growth-promotion and antibiosis of Bacillus amyloliquefaciens L-H15 was studied. Genes which were relevant to the characterizations of growth-promotion and antibiosis of B. amyloliquefaciens L-H15 were analyzed based on the complete genome sequencing. Meanwhile, the capabilities of biofilm formation, nitrogen fixation, phosphate and potassium solubilization, production siderophores and phytohormones were measured. The results indicated that L-H15 showed the capability of biofilm formation and the amount of bacteria which could colonize the root surface was up to 105CFU/g. The relative expression of siderophores was 60.22%, and the production of IAA, CTK and GA could reach 61.35pmol/L, 48.93ng/mL and 82.46pmol/L, respectively. The diameters of inhibition zone were (8.67±1.15)mm and (2.50±0.50)mm, respectively, when cocultured L-H15 with two fugal pathogens, i.e., Rhizoctonia solani and Fusarium oxysporum. In conclusion, L-H15, which showed excellent characterizations in both aspects of growth-promotion and antibiosis, had great potential to become a biological control agent in the future.