Mustard biomass and heavy metal enrichment and transfer characteristic under the separate furrow irrigation was studied, which plays an important role in the phytoremediation of soil heavy metals pollution. The experiment was conducted from March, 2014 through November, 2015, the irrigation frequency (I3, I4 and I5 were irrigated 3, 4 and 5 times, respectively, and the irrigation amount was 0.15m) and frequency of fertilizer (base fertilizer was 0.74g/m3, fertilizer amount was 1.3g/m3, according to water consumption of 0.01 m, according to frequency of F0, F1, F2, F3 and F4) were set up as two factors. Results showed that under the influence of irrigation frequency, mustard leaves of super oxide dismutase (sod) activity and proline were increased with the increase of irrigation frequency, malondialdehyde was on the decline. Biomass, cadmium mass fraction, cadmium enrichment coefficient, cadmium total accumulated amount of cadmium of I4 was increased by 12.33%~89.71%, 5.00%~44.33%, 0.50%~55.36% and 22.22%~114.81%, and those of I5 were increased by 19.21%~87.37%, 24.00%~93.51%，13.44%~112.30% and 77.42%~168.75%, respectively, compared with those of I3. The growth of biomass in the aboveground was greater than that of the root, but the root of the cadmium mass fraction, the cadmium concentration coefficient and the total amount of cadmium accumulation were greater than the ground. In the influence of fertilization frequency, mustard of biomass, cadmium mass fraction, cadmium total cadmium accumulation and cadmium enrichment coefficient under high frequency fertilization (F4) were increased by 22.55%~99.71%, 30.68%~87.40%, 37.80%~112.20% and 71.43%~213.51% than those under low frequency fertilization (F1), respectively, the roots growing rate was greater than the grounds in low frequency irrigation, and the middle and higher frequencies of irrigation the ground was lower than the roots. In addition, the cadmium transfer coefficient was decreased slightly with the increase of supply of water and fertilizer, but the overall maintenance level was high. Through model simulation and verification, the result showed that the ability of mustard heavy metal repair to be consistent with the frequency response of water and fertilizer.