江连洲,张巧智,关嘉琦,巩涵,李杨,隋晓楠.羧甲基化大豆水酶法膳食纤维的制备及其性能研究[J].农业机械学报,2017,48(10):330-337.
JIANG Lianzhou,ZHANG Qiaozhi,GUAN Jiaqi,GONG Han,LI Yang,SUI Xiaonan.Preparation of Dietary Fiber from Enzyme-assisted Aqueous Extraction of Soybeans through Carboxymethylation and Its Functional Properties[J].Transactions of the Chinese Society for Agricultural Machinery,2017,48(10):330-337.
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羧甲基化大豆水酶法膳食纤维的制备及其性能研究   [下载全文]
Preparation of Dietary Fiber from Enzyme-assisted Aqueous Extraction of Soybeans through Carboxymethylation and Its Functional Properties   [Download Pdf][in English]
投稿时间:2017-02-20  
DOI:10.6041/j.issn.1000-1298.2017.10.042
中文关键词:  大豆  膳食纤维  水酶法  羧甲基化修饰  响应面分析
基金项目:国家重点研发计划项目(2016YFD0401402、2016YFD0400700)、国家自然科学基金重点项目(31430067)、国家自然科学基金面上项目(31571876)和东北农业大学大学生创新创业训练项目(201610224178)
作者单位
江连洲 东北农业大学 
张巧智 东北农业大学 
关嘉琦 东北农业大学 
巩涵 东北农业大学 
李杨 东北农业大学 
隋晓楠 东北农业大学 
中文摘要:大豆水酶法残渣可作为膳食纤维的来源。以大豆水酶法残渣为原料,对其中的膳食纤维进行羧甲基化修饰,在单因素试验的基础上,通过Box-Behnken响应面试验设计建立了碱化温度、氯乙酸添加量、醚化时间和醚化温度对羧甲基取代度的模型。研究结果表明,各参数对羧甲基取代度的影响程度依次为醚化时间、氯乙酸添加量、碱化温度、醚化温度。通过对试验结果的二次回归分析得到的最佳制备工艺为碱化温度25℃、氯乙酸添加量为膳食纤维质量的1.05倍、醚化温度70℃、醚化时间3.8h,在此最优条件下制备的水酶法膳食纤维羧甲基取代度为0.4305,其持水率、持油率和膨胀率分别较修饰前提升了89.61%、20.63%和114.32%。红外光谱结果表明,制备的大豆水酶法膳食纤维发生了羧甲基取代反应。
JIANG Lianzhou  ZHANG Qiaozhi  GUAN Jiaqi  GONG Han  LI Yang  SUI Xiaonan
Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University,Northeast Agricultural University and Northeast Agricultural University
Key Words:soybeans  dietary fiber  enzyme-assisted aqueous extraction method  carboxymethylation modification  response surface analysis
Abstract:Enzyme-assisted aqueous extraction (EAEP) is an environmental-friendly method which is an alternative to conventional hexane oil extraction. During EAEP of soybeans, an additional by-product, residue fraction was produced. About 4.4kg residue would be produced for every liter soybean oil extracted. The residue fraction of EAEP of soybeans contains substantial amount of fibers, which can be considered as a rich resource of dietary fiber. A soybean dietary fiber was prepared from EAEP residues and further modified through carboxymethyl substitution. According to the results of single factor experiments, Box-Behnken response surface methodology was conducted to investigate the correlation between operating parameters and degree of substitution (DS). The results indicated that the effects of four parameters on DS followed an order of etherification time, chloroacetic acid dosage, alkalization temperature, and etherification temperature. For optimizing the four independent variables, square regression analysis was performed. The regression model was fitted with determination coefficient R2 of 0.9684 and optimal parameters were as follows: alkalization temperature was 25℃;dosage of chloroacetic acid was 1.05 times of the mass of raw fiber;etherification temperature was 70℃ and etherification time was 3.8h. The DS under above conditions was 0.4305. Compared with the raw sample, the water holding capacity, oil holding capacity and expansion capacity of modified fiber were 8.21g/g, 3.80g/g and 9.88mL/g, which were improved by 89.61%, 20.63% and 114.32%, respectively. The infrared spectrum showed that carboxymethyl substitution was occurred during modification. The modified dietary fiber from EAEP of soybeans exhibited desired functional properties, which can be used in meat, beverage and bakery products.

Transactions of the Chinese Society for Agriculture Machinery (CSAM), in charged of China Association for Science and Technology (CAST), sponsored by CSAM and Chinese Academy of Agricultural Mechanization Science(CAAMS), started publication in 1957. It is the earliest interdisciplinary journal in Chinese which combines agricultural and engineering. It always closely grasps the development direction of agriculture engineering disciplines and the published papers represent the highest academic level of agriculture engineering in China. Currently, nearly 8,000 papers have been already published. There are around 3,000 papers contributed to the journal each year, but only around 600 of them will be accepted. Transactions of CSAM focuses on a wide range of agricultural machinery, irrigation, electronics, robotics, agro-products engineering, biological energy, agricultural structures and environment and more. Subjects in Transactions of the CSAM have been embodied by many internationally well-known index systems, such as: EI Compendex, CA, CSA, etc.

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